block diagram for glucose syrup production from tapioca strach
Release Time：2020-07-02 20:12Author：sd888Source：未知
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Overview Fructose syrup is a new type of sweetener that has developed rapidly at home and abroad in recent years. Both domestic and foreign use pure starch as raw material. After hydrolysis by a-amylase and saccharification enzyme, glucose is finally produced, and then solid-phase glucose isomerization is used. Enzymes areomerized to produce fruit glucose syrup. This sugar contains about 42 gut fructose, called the first generation of fructose syrup. Abbreviations abroad (HFCs), sweetness is similar to sucrose, flavor and metabolic pathways are similar to sucrose, so it is an ideal nutritional sweetener. The process using starch has low production efficiency and high product cost. Directly using high-yield cassava in the southern provinces for production, it can be directly made into starch enamel, with high raw material utilization rate, low unit consumption, low cost and simple process. The elimination of many starch-making processes opens a shortcut for township enterprises to develop and utilize local resources. Using isomerase, the fermentation activity is 40oD/, l, the self-made solid-phase enzyme activity is generally about g000D/ml, the conversion rate per gram of dry drunk is 42%, and the fruit slush is 4.1-5.3kg (equivalent to 4.1~5.3 ). Prepare the culture medium, inoculate a shaker bottle to get drunk, filter, remove residues, bacterial autolysis enzyme solution * centrifuge to remove the slime, supernatant 290-resin'Ting Zhai Ye Fufu Yi (Lin)-wash enzyme work 3. Isomerization part',._. ,, _t purification, _. ..: Tian, liquid enamel, bleaching, male pad Gong Yi, ingredients to adjust the side of the intestine. ’Ion exchange r is better than bamboo, PH-isomerization column, secondary purification, concentration-glucose frit. 3. Pumping process operation 1. Adjust the pulp, add a little cassava powder into milky form 19-20Be and mix well, adjust it to pH 6.0-6.2 with 5 sodium carbonate (soda ash), and add them separately. .3 Intestinal calcium chloride and the required α-amylase. The dosage is 80 units per gram of pure starch. Amylase should be mixed thoroughly with a small amount of cold water. The amount of starch per gram of raw material x the number of raw materials, the actual amount of a-amylase two (g) amount (g)_x the number of enzyme units required per gram of starch (soD/g) Second, the production process flow of a-amylase per gram of bacteria Total number of units (D) 1. Sugar-making cassava chips, crushed and soaked, liquefied and filtered, dross removal'decolorization' ion exchange, concentration, crystalline sugar 1 liquid sugar 2. Enzyme making part 2. Liquefaction: The purpose of liquefaction is to put The starch macromolecules become small molecules, the original viscosity starch reduces the viscosity, prepares a good substrate for the role of saccharification enzymes, the starch is pumped into the digester and heated to 90-94, after 5 minutes of liquefaction, it is sent to keep warm at 7010 minutes, After a-amylase is fully liquefied until the iodine color reaction is brownish yellow, after liquefaction, it is heated and boiled for 3 to 5 minutes to kill the enzyme. Open the jacket of the cooler to cool the cooling water, so that the temperature of the tuck liquid at the outlet drops to about 70. 3. Enamelization, pump the liquefied liquid into a thermally insulated saccharification tank equipped with a stirrer, add industrial hydrochloric acid to adjust the pH to 4, add Xiyixinhua drunk, the dosage is 80 units per gram of dry starch (same as a starch drunk) Calculation), the reaction temperature should be maintained at 60-72, the reaction time is 3 hours, the temperature is too low up to 48 hours, the DE value reaches 95 or more is the end of saccharification, boiling the enzyme at atmospheric pressure to kill the enzyme. 4. Decolorize and filter, add the saccharified liquid into the activated charcoal based on dry matter juice l 1.5 intestines, decolorize at 80 for 30 minutes, and then filter with a plate and frame filter to obtain a light transparent liquid. When the sugar liquid is pressure-filtered in the filter press, the lining fluid that begins to flow out is unclear, and it should be refluxed until the lining fluid is clear. 5. Ion exchange, the purpose is to remove ionic metal impurities (such as Mg+, Ca++, Fe++, etc.) and remove ionic pigments, the exchange system is carried out according to yang, yin, yang, and yin. During the exchange, the cationic resin 732 type, the pH rises from 2 to 4 is the exchange termination, the anion resin 701, the pH drops from 7 to 4 (or color difference) is the exchange end point. During the exchange, the sugar liquid flows from the top to the bottom through the ion exchange resin column. The flow rate of the sugar liquid is about 3 to 4 times the volume of the ion exchange resin per hour, and the temperature is controlled between 40 and 50. The resin leaching (regeneration) cationic resin 732 was soaked in 5-intestine HCI for 2 hours, and then rinsed thoroughly with clean water until the rinse water contained no ions, and the pH was 2 for use. The anionic resin 701 was soaked in 4-intestine Na0H for 2 hours, and then washed with clean water until the pH was about 7 for use. 6. The concentrated liquid can be concentrated to a concentration of 40~42 intestines at a vacuum of 600 μg and temperature of eo~65. The required conversion value. Generally, 15-2 tons of fructose syrup (dry matrix) can be produced per kilogram of solid phase enzyme, and the conversion rate is 45 gut, which is then filtered, decolorized by activated carbon, purified by ion exchange resin, and the slurry is concentrated by vacuum to 70-71 gut. The finished product. 2. Isomerase column preparation The isomerase column is to immobilize the enzyme, and then install the solid phase enzyme in the upright
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